C Figure four. IGF1 immunostaining, image evaluation by application in which the red color represents the count pixel2) with statistical analysis (pvalues in the table). For information, see the text. The information are count pixel2) with statistical evaluation (p-values in the table). For details, see the text. The information are immunolabelling (inserts), and also a graph representing the intensity of Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) Proteins Accession immunostaining (densitometric presented as mean SD. Scale bars: 50 m. presented as imply SD. Scale bars: 50 . count pixel2) with statistical evaluation (pvalues in the table). For details, see the text. The information are presented as mean SD. Scale bars: 50 m.Figure 5. DKK1 immunostaining, image analysis by software program in which the red color represents the immunolabelling (inserts), and a graph representing the intensity of immunostaining (densitometric Figure 5. DKK1 immunostaining, image analysis by computer software in which the red color represents the count pixel2) with statistical analysis (pvalues in the table). For specifics, see the text. The data are Figure 5. DKK-1 immunostaining, image analysis by computer software in which the red color represents the immunolabelling (inserts), in addition to a graph representing the intensity of immunostaining (densitometric presented as imply SD. Scale bars: 50 m. immunolabelling (inserts), andanalysis (pvalues in the table). For specifics, see the text. The data are a graph representing the intensity of immunostaining (densitometric count pixel2) with statistical count pixel2) with statistical evaluation (p-values within the table). For particulars, see the text. The information are presented as imply SD. Scale bars: 50 m.presented as mean SD. Scale bars: 50 .Nutrients 2018, 10,Nutrients 2018, 10,10 of10 of3.five.four. VDR In muscle fibers, VDR immunostaining was mainly cytoplasmic and, in some samples, nuclear. In muscle fibers, VDR immunostaining was primarily cytoplasmic and, in some samples, nuclear. The intensity of VDR immunostaining (densitometric count-pixel2) was higher in R, R-DS, HFB-DS, The intensity of VDR immunostaining (densitometric countpixel2) was greater in R, RDS, HFBDS, and HFEVO-DS groups. In detail: in R, the immunostaining was higher than in R-DR, HFB-DR, and HFEVODS groups. In detail: in R, the immunostaining was greater than in RDR, HFBDR, Death-Associated Protein Kinase 1 (DAPK1) Proteins Accession HFEVO-DR (p 0.01); in R-DS, it was larger than in R-DR, HFB-DR, HFEVO-DR (p 0.01); in R-DR, HFEVODR (p 0.01); in RDS, it was greater than in RDR, HFBDR, HFEVODR (p 0.01); in RDR, it was reduce than in HFB-DS, HFB-DR, HFEVO-DS, HFEVO-DR (p 0.01); in HFB-DS, it was higher it was reduced than in HFBDS, HFBDR, HFEVODS, HFEVODR (p 0.01); in HFBDS, it was greater than in HFB-DR, HFEVO-DR (p 0.01); in HFB-DR, it was lower than in HFEVO-DS (p 0.01); than in HFBDR, HFEVODR (p 0.01); in HFBDR, it was reduced than in HFEVODS (p 0.01); in HFEVO-DS, it was larger than in HFEVO-DR (p 0.01) (Figure 6). In relation for the immunostained in HFEVODS, it was higher than in HFEVODR (p 0.01) (Figure six). In relation towards the immunostained location , the statistical benefits were analogues to these from the intensity of VDR immunostaining (information area , the statistical final results have been analogues to those of the intensity of VDR immunostaining not(data not shown). shown).3.five.four. VDRFigure six. VDR immunostaining, image evaluation by application in which the red color represents the Figure 6. VDR immunostaining, image ana.