The production of drug-loaded EVs and to discover feasible application for in situ drug delivery program. Funding: This analysis is funded by Focused Ultrasound Foundation.OS23.Extracellular Vesicles for new Molecular Insight to Biomolecular Interactions Tamas Beke-Somfaia, Priyanka Singhv, Imola Szigyarto and Zoltan VargacaPI, Budapest, Hungary; bMs, Budapest, Hungary; cResearch Centre for Organic Sciences, Hungarian Academy of Sciences, Budapest, HungaryIntroduction: The prospective of extracellular vesicles (EVs) to revolutionize the diagnosis and therapy of different diseases has been realized and as a result it is actually an extensively studied path. Even so, EVs are also in the size range appropriate for membrane biophysics, even though they preserve the complex composition of a biological bilayer. Consequently, they’re optimal for monitoring the structure, orientation and function of biomolecules related to EVs.Approaches: The investigated red blood cell-derived vesicles (REVs) were isolated from blood making use of a normal protocol and purified applying size-exclusion chromatography. REVs had been subjected to IR, CD and flow-Linear Dichroism spectroscopy, freeze-fracture Transmission Electron Microscopy also as Dynamic Light Scattering. Final results: Here we demonstrate that polarized light spectroscopy tactics can supply vital details on REVs and molecules inserting into their bilayer. Flowlinear dichroism (flow-LD) measurements show that EVs is usually oriented by shear force, insight into properties of oriented macromolecules inside the vesicles. The Soret-band on the LD spectra demonstrates that hemoglobin molecules are oriented and related for the lipid CD40 Ligand/CD154 Proteins Recombinant Proteins bilayer in freshly released REVs [1]. Additional on, we selected 3 distinctive antimicrobial peptides (AMPs), CM15, melittin and gramicidin and investigated their interactions with REVs employing a 4-1BB/CD137 Proteins Formulation diverse set of tactics. The peptide-membrane interactions reveal a number of novel function of AMPs, such as their capability to take away associated proteins in the surface of REVs (Figure 1). [1] I. Cs. Szigy t R. De , J. Mih y, S. Rocha, F. Zsila, Z. Varga, T. Beke-Somfai. Flow-alignment of extracellular vesicles: structure and orientation of membrane associated biomacromolecules studied with polarized light. ChemBioChem. 2018;19:54551 Summary/Conclusion: In conclusion, EVs present superb possibilities to superior recognize the function and mechanism of all-natural membrane active biomolecues. Funding: This function was funded by the Momentum programme (LP2016-2), by the National Competitiveness and Excellence System (NVKP_16-1-20160007) and BIONANO_GINOP-2.three.2-15-2016-00017. The J os Bolyai Research Scholarship (Z.V.) is considerably acknowledged.JOURNAL OF EXTRACELLULAR VESICLESSymposium Session 24: Mechanisms of EV Delivery Chairs: Pieter Vader; Hang Hubert Yin Location: Level B1, Hall B 13:004:OS24.State of the art microscopy for live cell study from the extracellular vesicle-mediated drug delivery Ekaterina Lisitsynaa, Kaisa Rautaniemia, Heikki Saarib, Timo Laaksonena, Marjo Yliperttulab and Elina Vuorimaa-Laukkanena Laboratory of Chemistry and Bioengineering, Tampere University of Technologies, Tampere, Finland; bDivision of Pharmaceutical Biosciences and Drug Analysis Program, Faculty of Pharmacy, University of Helsinki, Helsinki, FinlandaSummary/Conclusion: This research provides new realtime procedures to investigate EV kinetics with living cells and complements the existing procedures. The findings from the study strengthen the.