Tients with diabetes. Strategies: Patients at Concord Hospital with suspected CAD gave written informed consent and were administered RIPC (sphygmomanometer around the arm, three 5 min cycles, n = 31) or sham (n = 29) just before angiography, with recruitment ongoing. Blood was collected pre- and straight away post-RIPC/sham and plateletfree plasma generated. International coagulation/fibrinolytic potential was measured by general haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and numerous fibrinolytic elements by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Analysis institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have possible as diagnostic and αvβ5 Accession prognostic biomarkers. In atherosclerosis, the underlying bring about of heart attack and stroke, EV release might be dysregulated and their contents can mediate pro-inflammatory effects. Various markers have already been previously identified on uEV like exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers contain microRNAs (miRs). miR-21 and miR-155 are important regulatory miRs that happen to be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to possess pro-atherogenic effects and miR-155 deficiency in murine models results in decreased atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from patients diagnosed with coronary artery illness (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic individuals have been TLR8 review isolated via benchtop centrifugation. The concentration and size of uEVs were analysed via the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = ten per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Final results: uEV concentration in symptomatic individuals (median; six.46E+9 particles/mL) was substantially decreased (p 0.05) in comparison to asymptomatic patients (median; 1.25E+10 particles/mL). CD11B+ uEVs had been enhanced and CD16+ uEVs had been decreased in the symptomatic individuals (p 0.01). Furthermore, the concentration of CD45+ EVs had been elevated in symptomatic sufferers (p 0.001). Though uEV miR-21 was unchanged, miR-155 expression was significantly enhanced inside the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic possible. As CAD severity increases, uEV concentration is lowered, surface marker expression is altered and uEV miR-155 expression is improved. Funding: The Irish Analysis Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal illness Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Wellness Evaluative Sciences, Analysis Institute, The Hospital for Sick Kids,.