Illic acid Caffeic acid Vanillin Syringaldehyde 4-Hydroxybenzeldehyde 4-Hydroxyacetophenone Osmolality (mol/kg)a ACSHACSH w/o autoclavingg 5.5 5.five 1.1 2.1 0.71 0.48 0.08 0.09 0.09 0.01 0.132 0.162 0.197 0.025 w/autoclaving three.5 0.six 7 1.three .1 0.03 1.4 0.3 0.091 0.003 0.32 0.01 0.036 0.004 0.15 0.02 0.006 0.001 0.24 0.04 0.017 0.002 0.15 0.02 0.017 0.SynH1a SynH2- 10 ten 0.010 0.025 0.400 0.003 16.six 12 one hundred ten ten 0.010 0.030 ten 0.003 17 12SynH2 10 ten 0.010 0.030 10 0.003 17 120.two.75 2.75 0.55 1.05 0.355 0.48 0.08 0.09 0.09 0.01 0.132 0.162 0.197 0.1.16 0.1.17 0.01 1.19 0.information are from Schwalbach et al. (2012). Sugar concentrations are aver-ages of HPLC-MS and NMR determinations.b Inthe SynH2 recipe, D-Arabinose was substituted for the L -Arabinose presentin ACSH to prevent AraC-mediated repression of xylose-utilization genes (Desai and Rao, 2010). In other contexts, use of L -Arabinose in SynH2 will be proper.c not determined in ACSH or not added in SynH. not detectable by methods applied. compounds detected at less than 20 in ACSH are not reportedd n.d.,e Aromatichere.f Thesets of acids, amides, and aldehydes made use of for supplemental research informulating SynH2 consisted of p-Coumaric acid, Ferulic acid, Benzoic acid, Syringic acid, Cinnamic acid, Vanillic acid, and Caffeic acid (acids), Feruloyl amide and Coumaroyl amide (amides), and HMF Vanillin, Syringaldehyde, 4, Hydroxybenzaldehyde, and 4-Hydroxyacetophenone (aldehydes) in the concentrations listed for non-autoclaved ACSH or fractions thereof as described inside the Supplemental Results.g ACSHInhibitor concentrations for non-autoclaved CS hydrolysate are from(Tang et al., submitted). Hydrolysate preparations are described in Materials and Solutions.(RSEM) version 1.two.four (Li and Dewey, 2011). Posterior mean estimates of counts and FPKM values have been utilized within the downstream evaluation. The plan edgeR v.three.0.two (Robinson et al., 2010) was utilised to compute differential expression by using the procedures and methods described inside the package documentation in all function calls with median normalization as an alternative to the default TMM procedure. We found that median normalization far better adjusted for the unique biases within the dataset. Adjusted p-values for several hypothesis corrections had been utilised as calculated by edgeR. PairwiseFrontiers in Microbiology | Microbial Physiology and MetabolismAugust 2014 | Volume five | Short article 402 |Keating et al.Bacterial regulatory responses to lignocellulosic inhibitorsTable 2 | Growth, sugar uptake, and ethanol production by GLBRCE1 grown in ACSH and SynH2- , and SynH2a . Media SynH2- TrkC Activator Formulation Development (Exponential) (hr-1 )b Glucose Price (Exponential)b Glucose Price (Transition)c Xylose Rate (Transition)c Glucose Price (Glu-Stationary)d Xylose Price (Glu-Stationary)d Xylose Rate (Xyl-Stationary)e Total Glucose Consumed (mM) Total Xylose Consumed (mM) Total Ethanol made (mM) Ethanol Yield ( )fa EachSynH2 0.09 0.02 five.9 1.3 two.6 0.4 0.5 0.1 1.6 0.two 0.11 0.05 0.01 0.01 310 20 25 1 460 60 70 ACSH 0.12 0.01 five.six 1.3 two.7 0.1 0.two 0.1 1.4 0.2 0.11 0.04 0.04 0.03 300 20 25 10 470 60 73 0.13 0.01 four.7 0.5 three.two 0.1 0.6 0.1 N/A N/A 0.19 0.03 330 20 65 30 540 30 70 worth is from at least three biological replicates in distinctive bioreactors. phase is between 4 and 12 h in all media. Unit for glucose uptakeb Exponential-1 price is mM D600 -1 . c Transitionphase is involving 12 and 30 h for SynH2-, and between 12 and23 h for SynH2 and ACSH. Units for glucose and xylose P2X1 Receptor Antagonist supplier uptake price are mM-1 D600 -1 . d.