Product Name :
PTEN (human), (recombinant) (His-tag)
Sequence:
Purity:
≥80% (SDS-PAGE)
Molecular Weight:
~48 kDa
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Use/Stability :
Description:
PTEN (phosphatase and tensin homologue deleted on chromosome 10, MMAC) is a lipid phosphatase which can also act as a tyrosine, serine and threonine protein phosphatase. It is specific for acidic substrates such as phosphatidylinositol(3,4,5)-trisphosphate, which is its main biological substrate that mediates growth factor-induced activation of intracellular signalling through the serine-threonine kinase Akt. The tumor suppressor gene, PTEN, also known as MMAC1 or TEP1, has been isolated from a locus on chromosome 1Oq23; this protein is deleted or mutated in a large number of tumors. Germline mutations of PTEN have been identified in Cowden disease, Lhermitte-Duclos disease, and Bannayan-Zonana syndrome.
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Solubility:
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Additional Information :
| Alternative Name Phosphatase and tensin homolog deleted on chromosome 10 | Application Notes Can be used to study regulation and kinetics of PTEN, screen for PTEN inhibitors or activators or as positive control for Western blots.{{128794-94-5} medchemexpress|{128794-94-5} Purity & Documentation|{128794-94-5} References|{128794-94-5} manufacturer} | Formulation Liquid.{{2672512-44-4} web|{2672512-44-4} Protocol|{2672512-44-4} In Vivo|{2672512-44-4} custom synthesis} In 50mM TRIS-HCl, pH 8.PMID:26247089 0, containing 150mM sodium chloride, 5mM DTT, 0.03% Brij 35, 0.1mM EDTA and 20% glycerol. | MW ~48 kDa | Purity ≥80% (SDS-PAGE) | Purity Detail Purified by multi-step chromatography. | Source Produced in E. coli. Full length human PTEN (aa 1-403) is fused at the N-terminus to a His-tag. | Specific Activity ≥ 7 pmol/min/µg assayed by Ptdlns(3,4,5)P3 (60 µM) hydrolysis at 37°C | UniProt ID P60484