The localization of DmManf inside the ER (Figure 3B) in third instar larval garland cells of homozygous DmManf mutants was considerably reduced when the putative ER retention signal was deleted (Figure 3B)
Right after incubation for seventy two h, V5-DmManf-Dss and DmManf-Dss were being detected in cell extracts but not in the medium of S2 or CHO cells by Western blotting (Determine…