Product: NH4-KLGADTDGEQDQHMTYGGQ-COOH

NELF-E Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human negative elongation factor polypeptide E using the numbering given in entry NP_002895.3 (GeneID 7936).
Clonality
Polyclonal
Host
Rabbit
Gene
RDBP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NELF-E Lysate (NBL1-15253)

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NELF-E Antibody

  • D6S45
  • negative elongation factor E
  • negative elongation factor polypeptide E
  • NELFE
  • NELF-ERDmajor histocompatibility complex gene RD
  • nuclear protein
  • RD RNA binding protein
  • RD RNA-binding protein
  • RDP
  • RNA-binding protein RD

Background

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq]

PMID: 18703661

Product: Aminohippurate (sodium)

NELF-E Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDG
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (93%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RDBP
Purity
Affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NELF-E Antibody

  • D6S45
  • negative elongation factor E
  • negative elongation factor polypeptide E
  • NELFE
  • NELF-ERDmajor histocompatibility complex gene RD
  • nuclear protein
  • RD RNA binding protein
  • RD RNA-binding protein
  • RDP
  • RNA-binding protein RD

PMID: 8393786

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