Oma mouse model. Summary/Conclusion: Our findings assistance the usage of allogeneic exosomes over syngeneic for therapeutic use in clinical studies where an adaptive immune response is preferred. Funding: This perform was supported by Swedish Medical Study Council, the Cancer and Allergy Foundation, the Swedish Cancer Foundation, along with the Radiumhemmets Research Foundations.NIMA Related Kinase 3 Proteins Recombinant Proteins Background: Exosomes show promise for the delivery of therapeutics as a result of their ability to provide higher levels of payloads by fusion with cells, but lack precise targeting to diseased cells leading to toxicities. RNA nanoparticles can especially target cancer cells but undergo endosome entrapment limiting their therapeutic influence. Right here advantages on the two technologies are combined to particularly delivery compact interfering RNAs (siRNAs) at a higher payload. Methods: Exosomes isolated from HEK293T cells have been purified by centrifugation with addition of a high density cushion to stop destruction from centrifugation forces. Arrow-shaped RNA nanoparticles containing cancer-targeting moieties had been decorated on exosome surfaces by hydrophobic cholesterol labels. siRNA was loaded into exosomes as payloads. Decorated exosomes were then tested against 3 cancer lines for therapeutic assessment. Benefits: It was shown that arrow shape from the RNA nanoparticles led to either internalization or surface show on exosomes. Putting the anchoring cholesterol on the arrow-tail outcomes in show of RNA aptamer or folate on the exosome surface. Putting the cholesterol in the arrow-head results in partial loading of RNA nanoparticles into the exosome. Resulting exosomes had been competent for precise delivery of siRNA, and effectively blocked tumour development in prostate cancer xenograft, orthotopic breast cancer and patient-derived colorectal cancer in vivo models. Final results show knockdown of survivin gene by siRNA delivery and no ADAM15 Proteins manufacturer indicators of toxicity. Summary/Conclusion: Here we combine the targeting positive aspects of RNA nanotechnology together with the delivery efficiency of exosomes overcoming roadblocks of each technologies, and offer an efficient strategy for ligand display to exosome for certain in vivo cell targeting. Reference: F Pi, et al, P Guo. Nanoparticle orientation to manage RNA loading and ligand display on extracellular vesicles for cancer regression. Nat Nanotechnol. 2018 Jan;13(1):829. Funding: The analysis was supported mostly by National Institutes of Health grants UH3TR000875 and U01CA207946 (to PG), and partially by R01CA186100 (to BG), R35CA197706 (to C.M.C.), P30CA177558 and R01CA195573 (to B. M.E.).OS24.Mesenchymal stem cell-derived extracellular vesicles delivered inside a thermosensitive gel are efficient healing mediators in porcine and murine models of digestive fistula Gabriel Rahmi1; Max Piffoux2; Jeanne Volatron3; Guillaume Perrod1; Laetitia Pidial4; Claire Wilhelm5; Olivier cl ent1; Florence Gazeau5; Amanda K A Silva5 Hopital Europ n Georges Pompidou, APHP and PARCC, INSERM U970, UniversitSorbonne Paris Cit(USPC), UniversitParis Descartes, Paris, France; 2Laboratoire Mati e et Syst es Complexes, Paris, France; 3 Laboratoire Mati e et Syst es Complexes, CNRS UMR 7047 UniversitParis Diderot, ten rue Alice Domon et L nie Duquet, France, France; four INSERM U970 – PARCC, PARIS, France; 5Laboratoire Mati e et Syst es Complexes, Paris, FranceOS24.RNA nanoparticle orientation to manage ligand display on exosomes for cancer regression Daniel W. Binzel1; Fengmei Pi1; Tae Jin Lee2; Zhefeng.