Eath. Subsequently, we created an experimental model in which bleomycin induces lung fibrosis, pulmonary arterial hypertension (PAH), and RV dysfunction in C57BL/6 mice. Human mesenchymal stem cells (hMSCs) retained in the lung release exosomes and ameliorate the myocardial Siglec-15 Proteins Recombinant Proteins ischaemia following coronary ligation. Here we hypothesize that hMSCs or their secretory activity (exosomes) may be employed to guard the RV/pulmonary arterial (PA) coupling for the duration of PAH in bleomycin-exposed mice. Strategies: We intravenously Type I IL-1 Receptor (IL-1R1) Proteins Recombinant Proteins administer hMSCs (500,000 cells), exosomes (20 /mouse) 30 and 35 days right after the recurrent (12 doses) instillation of bleomycin (20 mg/kg) into C57BL/6 mice. Subsequently, we performed haemodynamic evaluations (in spontaneously breathing mice) to assess the effect of these interventions on the RV function and PASaturday, 05 Maypressure of bleomycin-treated mice. We also evaluated the effects of MSCs and exosomes, around the RV adenosine triphosphate (ATP) generation, and ROS production. Outcomes: Compared to handle, bleomycin remedy induced important increases in RV systolic pressure (RVSP) (20 three vs. 32 1 mmHg) and RV diastolic stress (RVDP) (3 1 vs. 8 1 mmHg), and depressed RV ejection fraction (EF) (60 vs. 30) 60 days just after bleomycin injection. These alterations were drastically (p 0.05) attenuated by the intravenous administration of hMSCs (RVSP 20 3, RVDP two 1 mmHg, EF 60) or their exosomes (RVSP 20 3, RVDP five 1 mmHg, EF 60). Bleomycin effects on RV had been related with significant (p 0.05) increases in mitochondria RV H2O2 generation (1 1 vs. five.5 1 mmol/min/mg) and reduction in ATP production (20 3 controls vs. five and 10 4 pmol/min/mg) after bleomycin administration. These changes have been drastically (p 0.05) modulated by administration of hMSCs (RV H2O2 generation 3 1 and 3 1 mmol/min/mg, ATP production eight 3 and 12 four pmol/min/mg) or exosomes (RV H2O2 generation 2 0.five and 2 1 mmol/min/mg, ATP production 11 3 and 12 1 pmol/min/mg). Summary/Conclusion: Similar to humans with extreme IPF, bleomycin exposure induces important RV dysfunction in C57BL/6 mice. This RV dysfunction is associated with significant mortality, enhance in RV mitochondrial ROS and reduced ATP generation. These haemodynamic and metabolic responses within the RV of bleomycin-treated mice are ameliorated by the IV administration of hMSCs or exosomes. Funding: This perform was supported by NIH grants R01HL110344 and R01HL114795.PS01.Endothelial colony-forming cell-derived exosomes attenuate pulmonary hypertension and hypoplasia in neonatal rats Flore Lesage1; Joanne Joseph1; Rajesh A Alphonse2; Arul Vadivel1; Chan e CyrDepauw1; Shumei Zhong1; Dylan Burger3; Mervin C Yoder4; Bernard Th audSinclair Centre for Regenerative Medicine, Ottawa Hospital Analysis Institute, Ottawa, Ottawa, Canada; 2Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Edmonton, Canada; 3Kidney Investigation Centre, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, Canada; 4Wells Center for Pediatric Research, Indiana University, Indianapolis, IN, US, Indianapolis, USAPS01.Exosomes derived from mesenchymal stem cells as a attainable therapy for osteoarthritis Maria Elisabetta Federica Palam; Simonetta Carluccio1; Daniele Reverberi2; Georgina Shaw3; Frank Barry3; Mary Murphy3; Chiara Gentili1 University of Genoa, Genova, Italy; 2Ospedale Policlinico San MartinoIRCCS per l’Oncologia, Genova, Italy; 3NUIG Galway, Galway, IrelandBackground: Osteoarthritis can be a pathological.