(yellow). the native ligand (green) along with the experimental ligand (yellow).The structures of OBP, OBP1, and OBP7 created up up of monomers, with OBP OBP The structures of OBP, OBP1, and OBP7 areare created of two two monomers, with and and every single every single getting six -helices and OBP7 possessing seven -helices, odorant odorant OBP1OBP1 possessing six -helices and OBP7 obtaining seven -helices, with the together with the binding binding pocket the center of center of a hydrophobic tunnel that runs by means of the dimeric pocket placed in placed inside the a hydrophobic tunnel that runs through the dimeric interface. interface. Even so, OBP4 is created up monomer. Within this investigation, the active pockets Even so, OBP4 is made up of a singleof a single monomer. In this investigation, the active pockets of odorant binding proteins had been identified by removing the BACE2 review ligands that had been previously linked towards the receptors before targeting these cavities. The experimental ligands all docked at the exact same pockets because the native ligands, validating the docking protocol adopted in this study. Figures 114 depict the 3D Cathepsin S Biological Activity interaction between OBPs and ligands, whereas TableInsects 2021, 12,17 ofof odorant binding proteins had been identified by removing the ligands that had been previously linked towards the receptors prior to targeting these cavities. The experimental ligands all docked in the same pockets because the native ligands, validating the docking protocol adopted within this study.Insects 2021, 12, x FOR PEER REVIEWTable 6. Active pockets on the 4 selected odorant binding proteins of A. gambiae. Active Pockets ALA62, LEU73, LEU76, SER79, HIS85, ALA88, MET89, GLY92, LYS93, ARG94, TRP114, PHE123; Table 7. The number PRO13, LEU17, CYS35, ILE75, PHE120,complexes. and variety of bonds for the OBD igand LEU124 THR2, GLN5, HIS29, LYS33, ALA52 Interacting Amino Acids inside the MET91, ARG94, GLU14, ALA18, LEU58, ALA62, SER79, MET84, ALA88, MET89,Active Pockets GLN116, PHE18 ofProteins OBP 1 OBP 7 OBP 4 OBPLigands -pinene linalool cis-sabinene hydrate citronellal verbenone bornyl acetate -phellandrene -terpinene sabinene -pinene myrcene p-cymene(a)OBP 1 OBP 7 OBP OBP four Leu76, Trp114, Phe123 3D interaction in between OBPs and ligands,Met89 Table 7 Phe120, Leu124 Ala88, whereas Ala52 Figures 114 depict the Leu73, Leu76, Ala88, Met89, and ligand interaction forms. Generally, the Met Ala88, Met91, proteins inprovides the active residues Lys93, Cys35, Phe120 Ala52 Trp114 89 teract with their ligands mostly by way of hydrophobic interactions including -alkyl and alkyl interactions (Figures 114). The interaction of the ligands revealed that Nil they Leu73, Ala88 Phe120 Phe123 bind to at the very least one receptor in the pocket Leu17, with the OBPs. The OBPs demonstrated cavity Phe120, Ala88, Met91, Leu73, affinity for Arg94, Trp114 Nil varyingLeu76, Ala88,certain ligands also asLeu124 variation within the variety of residues inPhe123 volved within the interactions. The crystallographic structure of OBP7 was favorably bound Met89, Lys93, Arg94, Phe120 Phe123 Ala52 to citronellal (-5.5 kcal/mol) and myrcene (-6.two kcal/mol) by way of residues inside the Trp114, Phe123 Cys35, Phe120 Phe123 Nil binding cavity; Leu17 (-helix 1), Phe120, Leu124 (-helix 7). and Cys35 (-helix two) Leu73, Leu76, Met89, Lys93, Trp114 Phe120 Ala88 Nil (Figure 11). Similarly, linalool (-6.two kcal/mol), citronellal (-6.1 kcal/mol), and myrcene Leu73, Met89, Lys93 ALA88 Nil (-5.8 kcal/mol) favorably interacted with OBP Phe120 residues Ala88, Met91 (-helix five), through Leu73, Ala88, Trp114 12). I