atase three xanthine dehydrogenase amylase alpha 1A matrix metallopeptidase 12 monoamine oxidase B cyclin dependent Bcl-2 Inhibitor web Kinase 6 fms-related receptor tyrosine kinase 3 solute carrier household 22 member 12 carbonic anhydrase 9 arachidonate 15-lipoxygenase AXL receptor tyrosine kinase carbonic anhydrase two Protein Class Kinase CYP3 Activator Compound Enzyme Enzyme Kinase Kinase Transporter Enzyme Kinase -Table two. Possible targets of p-synephrine. No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Uniprot ID P18031 P31749 P14679 P03372 P11511 P14416 P08069 P00734 P14780 P10275 P21397 P09917 O14746 P05177 P12931 P11474 P08253 P30542 P15121 P00533 P14061 P35869 P35372 Gene PTPN1 AKT1 TYR ESR1 CYP19A1 DRD2 IGF1R F2 MMP9 AR MAOA ALOX5 TERT CYP1A2 SRC ESRRA MMP2 ADORA1 AKR1B1 EGFR HSD17B1 AHR OPRM1 Relevance Score 12.186 11.022 ten.667 10.026 9.307 8.109 7.564 6.902 6.393 5.830 5.226 five.000 4.864 four.092 four.045 3.994 3.992 three.818 3.658 3.653 3.553 three.423 3.300 Target protein tyrosine phosphatase non-receptor kind 1 AKT serine/threonine kinase 1 tyrosinase estrogen receptor 1 cytochrome P450 family 19 subfamily A member 1 dopamine receptor D2 insulin-like growth element 1 receptor coagulation issue II, thrombin matrix metallopeptidase 9 androgen receptor monoamine oxidase A arachidonate 5-lipoxygenase telomerase reverse transcriptase cytochrome P450 household 1 subfamily A member two SRC proto-oncogene, non-receptor tyrosine kinase estrogen-related receptor alpha matrix metallopeptidase two adenosine A1 receptor aldo-keto reductase loved ones 1 member B epidermal development aspect receptor hydroxysteroid 17-beta dehydrogenase 1 aryl hydrocarbon receptor opioid receptor mu 1 Protein Class Kinase Enzyme Nuclear receptor Enzyme G-protein coupled receptor Kinase Enzyme Enzyme Nuclear receptor Enzyme Enzyme Enzyme Kinase Nuclear receptor Enzyme G-protein coupled receptor Enzyme Kinase Enzyme Transcription element G-protein coupled receptorBiomolecules 2021, 11,8 of3.1.2. Construction of PPI Network To additional explore the interaction involving the possible targets, 53 hispidulin antiobesity prospective targets and 23 p-synephrine anti-obesity prospective targets have been put into the STRING database. The PPI networks had been placed inside the Cytoscape application for a visualization and analysis (Figures 2 and three). The 3 parameters, (1) degree, (two) betweenness centrality, and (three) closeness centrality, were applied to analyze the PPI networks. These three parameters indicate the importance and influence on the node inside a complicated network. The degree (degree centrality) is defined because the quantity of connections owned by a node [54]. Therefore, it’s probably the most simple and most intuitive indicator in the importance of a node within the network. The betweenness centrality measures the extent to which a node plays a bridging function inside a network. Precisely, it measures the node falls on the shortest path involving other pairs of nodes within the network [55]. The closeness centrality is related for the distance amongst nodes. It really is calculated because the typical in the shortest path length in the node to just about every other node within the network [56]. The nodes within the networks represent the target genes, and also the edges symbolize the connections between target genes. The size and colour of a node indicates the intensity of the degree. Therefore, the greater the degree from the target, the bigger the node, and also the color progressively deepens from yellow to red. The width on the edge designates the grade on the correlation among the targets; the bigger the c