Pigment through thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and
Pigment by means of thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1 Htransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1HNMR) analyses revealed the presence of antimicrobial pigment rodiginine NTR2 medchemexpress derivatives NMR) analyses revealed the presence of antimicrobial pigment rodiginine derivatives in Streptomyces sp. BSE6.1 [25]. Nevertheless, the genome analysis of strain BSE6.1 reveals the in Streptomyces sp. BSE6.1 [25]. Even so, the genome evaluation of strain BSE6.1 reveals the presence of an undecylDDR1 Formulation prodigiosin gene cluster which can be responsible undecylprodigiosin presence of an undecylprodigiosin gene cluster that is accountable forfor undecylprodigiproduction. For that reason, the the red red fraction of Streptomyces strain BSE6.1 [25] to become osin production. Consequently,otherotherfraction of Streptomyces strain BSE6.1 [25] is yet is however 13 elucidated and and identified by means of LC-MS, 13C NMR, HSQC, HMBC, and COSY data to be elucidated identified by way of LC-MS, C NMR, HSQC, HMBC, and COSY information to confirm the production of undecylprodigiosin or connected derivatives. to confirm the production of undecylprodigiosin or associated derivatives. Prior studies reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], Previous studies reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], and Streptomyces variegatus produce prodigiosin [16] (Table 1). Nevertheless, some strains of and Streptomyces variegatus create prodigiosin [16] (Table 1). Even so, some strains of Streptomyces coelicolor produce either undecylprodigiosin [17,20,58] or a mixture of prodigStreptomyces coelicolor create either undecylprodigiosin [17,20,58] or perhaps a mixture of prodiinine derivatives [59] (Table 1). Comparable to S. coelicolor [17,20,58,59], the first fraction of ginine derivatives [59] (Table 1). Equivalent to S. coelicolor [17,20,58,59], the first fraction of red pigment eluted from Streptomyces strain BSE6.1 by way of TLC revealed the presence red pigment eluted from Streptomyces strain BSE6.1 by means of TLC revealed the presence of of methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry analysis but identified it as prodigiosin in 1 H NMR analysis [25]. Methyl-3-propyl prodiganalysis but identified it as prodigiosin in 1H NMR evaluation [25]. Methyl-3-propyl prodiinine and 2-methyl-3-butyl prodiginine had been also identified in actinomycetes [60], nonginine and 2-methyl-3-butyl prodiginine were also identified in actinomycetes [60], nonactinomycetes bacteria including Pseudoalteromonas rubra [61], and Serratia marcescens [62]. actinomycetes bacteria for example Pseudoalteromonas rubra [61], and Serratia marcescens [62]. These studies suggest that some strains of Streptomyces produce either prodigiosin or These studies recommend that some strains of Streptomycesof prodiginine analogs. undecylprodigiosin, whereas some make a mixture produce either prodigiosin or undecylprodigiosin, whereas someof strain BSE6.1 made a total of 7,528,288 reads. AssemWhole-genome sequencing make a mixture of prodiginine analogs. bling these raw reads resulted inside a single scaffold of eight.02 Mb with no extra-chromosomal content. Annotating the assembled genome of strain BSE6.1 indicated the presence of at the very least 7157 protein-coding genes, 82 tRNA coding genes, 3 rRNA coding genes, and.