Heir relative abundances.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author
Heir relative abundances.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure ten.Photos of VGLUT2 immunolabeled 5-HT1 Receptor Storage & Stability synaptic terminals in rat striatum ending on D1 spines (A,C), D1-negative spines (B,D), D1 ACAT Storage & Stability dendrites (E), or D1-negative dendrites (F). Spines (Sp) have been recognizable by their little size, the presence of spine apparatus, and also the absence of mitochondria (M) and microtubules, although dendrites (De) were recognizable by their bigger size, the presence of mitochondria and microtubules, plus the absence of spine apparatus. VGLUT2 synaptic terminals formed asymmetric synaptic contacts, asJ Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Lei et al.Pagerecognizable by the thick postsynaptic density (PSD). All pictures are at the exact same magnification as shown in (F).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 11.Graphs showing the size frequency distributions of VGLUT2 axospinous (A) and axodendritic (B) synaptic contacts on D1 and D1-negative spines and dendrites in striatum, graphed as a function of spatial frequency per terminal kind of a offered size. Note that VGLUT2 contacts on D1 spines and den-drites are a lot more common than on D1-negative spines and den-drites, and also the important distinction appears to become inside the greater abundance of smaller terminals on the D1 structures.J Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 12.Graphs showing the size frequency distributions for axospinous synaptic input to striatonigral (A) and striato-GPe neurons (B) in rats. For each neuron types we employed prior info around the kinds of cortical axospinous inputs (IT and PT) to these two neuron sorts, the size frequency distributions for these two cortical input kinds, the size frequency distribution for axospinous terminals on retrogradely labeled striatonigral and striato-GPe neurons, as well as the present findings on thalamic input to these striatal neuron sorts to derive estimates on the relative abundance of every single input type towards the two striatal projection neuronJ Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.Pagetypes (Lei et al., 2004; Reiner et al., 2010). Note that 62.7 IT plus a 37.three thalamic input yields a really close size frequency distribution match for striatonigral neurons. In the case of striato-GPe neurons, 54.two PT, 20 IT and 25.eight thalamic yields a close approximation for the axospinous input to this neuron sort.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; offered in PMC 2014 August 25.TABLELei et al.Antibody InformationType and host Guinea pig polyclonal AB5905 GATHSTVQPPRPPPPVRDY Guinea pig polyclonal AB5907 VQESAQDAYSYKDRDDYS 1:five,000 (EM) 1:1,000 (LM) Millipore Chemicon Synthetic peptide from rat VGLUT2 C-terminus (amino acids 56582): 1:5,000 (EM) 1:1,000 (LM) Millipore Chemicon Synthetic peptide from rat VGLUT1 C-terminus (amino acids 54260): Supply Catalog number Antigen Dilution usedAntibodyVesicular glutamate transporter 1 (VGluT1)Vesicular glutamate transporter 2 (VGluT2)Vesicular glutamate transporter 2 (VGluT2) Rabbit polyclonal HEDELDEETGDITQNYINY Rat monoclonal LCPATNNAIE-TVSINNNGAA-MFSSHHEPRGSISKE.