RI and PmeI and cloned into EcoRI/PmeI digested pYSGR-JFH-1 backbone
RI and PmeI and cloned into EcoRI/PmeI digested pYSGR-JFH-1 backbone to yield pRluc-HCVsg 2a(JFH1), the sequence of which was confirmed by sequencing. Site-directed mutagenesis was performed around the HCVsg 1b(con1)…