With 1 mol/l Fura-2/AM (Beyotime, China) for 35 min, and then detected with a spectrofluorometer (F-4500FL, Hitachi High-technologies, Japan). The basal emission was measured by stimulating the cells with 340/380…
Gulated and 25 probesets (16 genes) were downregulated in the high CINGEC group. As expected, many genes implicated in aneuploidy and DNA damage response were over-expressed in high CIN samples.…
Nt from conventional HMGA. This conclusion is further supported by bioinformatic prediction of disordered sequences with the Predictor Of Naturally Disordered Regions (PONDR) software , showing that XHMG-AT-hook1 has only…
Dified Eagle's medium (DMEM; WAKO) containing 10 fetal bovine serum (FBS). Nonastroglial cells were removed by shaking on the following day, and the remaining cells were grown further for 3…
Ells. Moreover we analysed the biological characteristics of the artificial construct and this enabled us to hypothesize why providing a three-dimensional cellular architecture is essential to recreate the unique functions…
L cells. Moreover, there was no evidence of any inflammatory cellular infiltrate present within the urothelium or the underlying bladder mucosa and the expression of the differentiation-associated plaque protein uroplakin…
Physical stimulation. Survival rates were recorded using a Kaplan-Meier survival curve and analyzed using a log-rank test, followed by a Holm-Sidak test for pairwise multiple comparisons (Sigma Stat 3.5).D: Equal…
Rized using western blot analysis. After blocking with 5 nonfat milk in PBST for 1 h at room temperature. The rabbit polyclonal antibody against NOB1 (1:10000, Abcam, Cat. #ab87151) or…
NiVec database (2011-11-21 release, http://www.ncbi.nlm.nih. gov/VecScreen/UniVec.html). For all contigs longer than 250 bp the open reading frames most likely to encode proteins were identified using the transcripts_to_best_scoring_ORFs.pl script distributed with…
E site of transcription initiation but does shift the site where the polymerase pauses . Pho could form part of the tethering complex when it binds close to the TSS…